Mouse Stem Cell (Oct4-GFP)

Cell Line: GOWT1 (Oct4-GFP) · Cell Migration / Developmental Biology

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01Microscopy Image

1 Channels · 1024 × 1024 px · 0.24 µm/px

GOWT1 Time-Lapse Cellular Migration Series

GOWT1 (Oct4-GFP)·fluorescence

Objective: 20x / 0.5 NA Plan-Neofluar · Zeiss Axiovert 200M Live Cell Station

100%

20 µm

Provenance HUD

Staining: Oct4-GFP Fusion Protein (Endogenous live reporter)

Treatment: Continuous live observation in conditioned medium

Resolution: 1024 × 1024 px (0.24 µm/px)

Accession: CTC-Fluo-N2DH-GOWT1 · Open Access Academic Research

Source: PMID: 28248386

02Acquisition & Provenance Metadata

MICROSCOPEZeiss Axiovert 200M Live Cell Station

OBJECTIVE20x / 0.5 NA Plan-Neofluar

PIXEL RESOLUTION0.24 µm / pixel

STAINING PROTOCOLOct4-GFP Fusion Protein (Endogenous live reporter)

EXPERIMENTAL TREATMENTContinuous live observation in conditioned medium

ACCESSION & LICENSECTC-Fluo-N2DH-GOWT1 · Open Access Academic Research

03Biological Context & Oncology Function

GOWT1 mouse embryonic stem cells express an Oct4-GFP reporter transgene. This standard time-lapse microscopy dataset from the Cell Tracking Challenge enables rigorous evaluation of cell division, nuclear morphology dynamics, and directional motility.

Lineage: Pluripotent embryonic stem cell

04Associated Genes (1)

Pou5f1▤ Supported by literature

Essential homeodomain transcription factor governing pluripotency and self-renewal.

Ref: MGI:101865

05Associated Proteins (1)

Octamer-Binding Transcription Factor 4□ Observed in image

Nuclear marker visualized in the GOWT1 reporter strain.

Ref: UniProt:P20263

06Biological Pathways (1)

Core Embryonic Stem Cell Pluripotency Circuit▤ Supported by literature

Transcriptional circuit regulating cellular differentiation and embryonic development.

Reactome: Reactome:R-MMU-452723

07Associated Diseases & Pathologies (1)

Embryonic Germ Cell Pathologies

Deregulated Oct4 expression correlates with malignant germ cell transformation.

08Phenotypic Profiles & Morphology Metrics

Centroid Migration (101.3 um)□ Observed in image

Quantified track 22 centroid persistence speed of 0.22 um/min over 455 minutes.

Model-Derived Nuclear Morphometry (StarDist2D + Constant Velocity Hungarian Tracker (Detection F1 0.917))

OBJECTS SEGMENTED61

MEAN CIRCULARITY0.884

MEAN ASPECT RATIO1.48

EVIDENCE LEVEL□ Observed

09Literature Evidence (1)

Ulicna et al. Nat Methods (2017)PubMed:28248386

An objective comparison of cell tracking algorithms across diverse multi-dimensional microscopy datasets.

▤ Supported by literature

10Dataset & Source Repository

Dataset Repository:Cell Tracking Challenge (CTC Fluo-N2DH-GOWT1)

Accession Identifier:CTC-Fluo-N2DH-GOWT1

Licensing Terms:Open Access Academic Research

Scientific Attribution:Cell Tracking Challenge / E.G. Reynaud et al.